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the present invention is directed toward a modified TetR(A) protein comprising an amino acid substitution at a position selected from the group consisting of positions 59, 70, 71, 91, 95, 96, 99, 100, 101, 103, 114, 127, 158, 159, 160, 179, 193, 197, and 201 of the TetR(A) protein as depicted in SEQ ID NO: 34, wherein said modified TetR(A) protein binds a TetR(A) operator sequence with greater affinity in the presence of tetracycline than in the absence of tetracycline; wherein: the amino acid substitution at position 59 is asparagine; the amino acid substitution at position 70 is valine; the amino acid substitution at position 71 is valine; the amino acid substitution at position 91 is glutamine; the amino acid substitution at position 95 is selected from the group consisting of glycine and glutamic acid; the amino acid substitution at position 95 is glycine; the amino acid substitution at position 95 is glutamic acid; the amino acid substitution at position 96 is arginine; the amino acid substitution at position 96 is glutamic acid; the amino acid substitution at position 99 is glutamic acid; the amino acid substitution at position 100 is alanine; the amino acid substitution at position 101 is histidine; the amino acid substitution at position 103 is serine; the amino acid substitution at position 114 is valine; the amino acid substitution at position 127 is selected from the group consisting of arginine, lysine, and histidine; the amino acid substitution at position 158 is cysteine; the amino acid substitution at position 159 is leucine; the amino acid substitution at position 160 is valine; the amino acid substitution at position 179 is valine; the amino acid substitution at position 193 is glycine; the amino acid substitution at position 197 is tryptophan; and the amino acid substitution at position 201 is histidine. Demographics, age of onset, relation to headache, additional visual symptoms, and past investigations were collected only in cases with a clear diagnosis of visual snow! Treatments may be limited to twice weekly and applied bifrontally or unilaterally to the non-dominant hemisphere to minimize confusion. • Keep the medication away from the corners of the nose, mouth, eyes, and open wounds. Sexually transmitted diseases treatment guidelines 2002! So fragrantly methocarbamol price
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užití obsahu včetně převzetí, šíření či dalšího zpřístupňování článků a fotografií je bez souhlasu MAFRA, a. Como no lo tenemos, trental nursing implications
vamos a prohibirlo", denuncia. 5,789,156, a Tn10-derived Tet repressor having amino acid substitutions at amino acid positions 71, 95, 101 and 102 has the desired functional properties and thus can be used as the first polypeptide in the transcriptional regulator fusion protein of the invention.
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